Hydrolyzed gelatin4/25/2023 ![]() It is a drying process where the solvent is crystallized at low temperatures and then sublimed from the solid-state directly into the vapor phase. doi: 10.1007/s11098-w.Freeze-drying (FD) is the most substantial drying technique utilized in the pharmaceutical and biopharmaceutical industries. Grand challenges in pharmaceutical research series: ridding the cold chain for biologics. ![]() Yu YB, Briggs KT, Taraban MB, Brinson RG, Marino JP. Lessons learnt from 12 oral cholera vaccine campaigns in resource-poor settings. Hsiao A, Desai SN, Mogasale V, Excler JL, Digilio L. Animal models for the study of human disease. Survival of enveloped and non-enveloped viruses on inanimate surfaces. doi: 10.1139/m75-121.įirquet S, Beaujard S, Lobert PE, Sané F, Caloone D, Izard D, Hober D. LRV: Log reduction value, * P value < 0.05 (Welch’s t test), HG: Hydrolyzed gelatin F Effect of three freeze–thaw cycles on BHV stability in hydrolyzed gelatin. The numbers indicate the molecular weight of samples, and A and B, acid-treated and alkaline-treated hydrolyzed gelatin respectively. E Stability of BHV in acid-treated and alkaline-treated hydrolyzed gelatin after 3 weeks of storage at 25 ☌. Zeta potential and pH of each molecular weight samples were showed in the figure. D Isoelectric points of varying acid-treated and alkaline-treated hydrolyzed gelatin used for virus stability formulations. C Stability of BHV in hydrolyzed gelatin liquid formulation with varying concentrations in 3- and 8-week storage at 25 ☌. B Stability of BHV in the hydrolyzed gelatin liquid formulation with varying concentrations in 3- and 8-week storage at 4 ☌. LRV: Log reduction value, ** P value < 0.01 (Welch’s t test), ns: non statistically significant. The results demonstrated that 5% of 4000 MW hydrolyzed gelatin formulation can act as a relevant stabilizer for the thermal stability of viruses in medical research and application.įormulation Hydrolyzed gelatin Stability Temperature Virus.Ī Stability over 3 weeks of storage of BHV-1 in the hydrolyzed gelatin liquid formulation at 4 ☌ and 25 ☌. All four viruses exhibited stability at 4 ☌ for at least 8 weeks, BHV or AdV remained stable for over 30 weeks of storage, and at 25 ☌, AdV and RV remained stable for 8 weeks. Based on the gelatin type, BHV in alkaline-treated hydrolyzed gelatin samples were unexpectantly more stable than in acid-treated hydrolyzed gelatin sample. The BHV model virus was considered stable after 3 weeks in hydrolyzed gelatin (MW: 4000) with a 0.8 LRV (log10 reduction value) at 25 ☌ or a 0.2 LRV at 4 ☌, compared to the stabilities observed in higher MW gelatin (60,000 and 160,000) with an LRV above 1. Using the model virus liquid formulation, the stability of multiple enveloped and nonenveloped RNA and DNA viruses, including parainfluenza virus, reovirus (RV), BHV, and adenovirus (AdV), was monitored over up to a 30-week storage period. The thermal stability of viruses in gelatin liquid formulations for medical research and application is poorly understood and this study aimed to examine the thermal stability of 4 enveloped and nonenveloped DNA and RNA viruses in hydrolyzed gelatin liquid formulations.īovine herpesvirus (BHV) was used as a model virus to examine the molecular weight (MW), concentration and gelatin type and to optimize virus stability in liquid formulations at 25 ☌ and 4 ☌.
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